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ev d68 strains us mo  (ATCC)


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    ATCC ev d68 strains us mo
    Ev D68 Strains Us Mo, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ev d68 strains us mo/product/ATCC
    Average 95 stars, based on 46 article reviews
    ev d68 strains us mo - by Bioz Stars, 2026-04
    95/100 stars

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    HEK-293T cells were transfected with siRNA pools targeting eIF4A3, Y14, MAGOH, or non-targeting control siRNAs (NC), respectively. At 48 hrs post-transfection, cells were inoculated with the designated virus (A) HPIV3, (B) Cedar, (C) MuV, (D) NDV, (E) Influenza A (A/WSN/1933), (F) Enterovirus <t>D68,</t> and (G) SARA-CoV2 at a multiplicity of infection (m.o.i.) of 0.01. The titers of infectious supernatants were determined on Vero-CCL81 cells using a 10-fold serial dilution at the indicated time points. For each virus, the expression levels of endogenous eIF4AIII, Y14, and MAGOH, along with infection control for viral protein or EGFP reporter, were analyzed by immunoblotting; results shown beside each panel confirm the knockdown of target proteins and validate virus infection. Symbols represent the data points from biological triplicates. Bars represent the mean of the triplicates. Statistical significance was determined by two-way ANOVA with Dunnett multiple comparison test. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001; ns, not significant. Immunoblottings are shown beside each to determine the knockdown of target proteins and controls for virus infection.
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    HEK-293T cells were transfected with siRNA pools targeting eIF4A3, Y14, MAGOH, or non-targeting control siRNAs (NC), respectively. At 48 hrs post-transfection, cells were inoculated with the designated virus (A) HPIV3, (B) Cedar, (C) MuV, (D) NDV, (E) Influenza A (A/WSN/1933), (F) Enterovirus <t>D68,</t> and (G) SARA-CoV2 at a multiplicity of infection (m.o.i.) of 0.01. The titers of infectious supernatants were determined on Vero-CCL81 cells using a 10-fold serial dilution at the indicated time points. For each virus, the expression levels of endogenous eIF4AIII, Y14, and MAGOH, along with infection control for viral protein or EGFP reporter, were analyzed by immunoblotting; results shown beside each panel confirm the knockdown of target proteins and validate virus infection. Symbols represent the data points from biological triplicates. Bars represent the mean of the triplicates. Statistical significance was determined by two-way ANOVA with Dunnett multiple comparison test. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001; ns, not significant. Immunoblottings are shown beside each to determine the knockdown of target proteins and controls for virus infection.
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    ev-d68 us/ mo/14-18947 (mo/47) strain  (Horng Gee Co Ltd)
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    HEK-293T cells were transfected with siRNA pools targeting eIF4A3, Y14, MAGOH, or non-targeting control siRNAs (NC), respectively. At 48 hrs post-transfection, cells were inoculated with the designated virus (A) HPIV3, (B) Cedar, (C) MuV, (D) NDV, (E) Influenza A (A/WSN/1933), (F) Enterovirus <t>D68,</t> and (G) SARA-CoV2 at a multiplicity of infection (m.o.i.) of 0.01. The titers of infectious supernatants were determined on Vero-CCL81 cells using a 10-fold serial dilution at the indicated time points. For each virus, the expression levels of endogenous eIF4AIII, Y14, and MAGOH, along with infection control for viral protein or EGFP reporter, were analyzed by immunoblotting; results shown beside each panel confirm the knockdown of target proteins and validate virus infection. Symbols represent the data points from biological triplicates. Bars represent the mean of the triplicates. Statistical significance was determined by two-way ANOVA with Dunnett multiple comparison test. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001; ns, not significant. Immunoblottings are shown beside each to determine the knockdown of target proteins and controls for virus infection.
    Ev D68 Us/ Mo/14 18947 (Mo/47) Strain, supplied by Horng Gee Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ev-d68 us/mo/14-18947 (mo/47) strain  (Horng Gee Co Ltd)
    90
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    HEK-293T cells were transfected with siRNA pools targeting eIF4A3, Y14, MAGOH, or non-targeting control siRNAs (NC), respectively. At 48 hrs post-transfection, cells were inoculated with the designated virus (A) HPIV3, (B) Cedar, (C) MuV, (D) NDV, (E) Influenza A (A/WSN/1933), (F) Enterovirus <t>D68,</t> and (G) SARA-CoV2 at a multiplicity of infection (m.o.i.) of 0.01. The titers of infectious supernatants were determined on Vero-CCL81 cells using a 10-fold serial dilution at the indicated time points. For each virus, the expression levels of endogenous eIF4AIII, Y14, and MAGOH, along with infection control for viral protein or EGFP reporter, were analyzed by immunoblotting; results shown beside each panel confirm the knockdown of target proteins and validate virus infection. Symbols represent the data points from biological triplicates. Bars represent the mean of the triplicates. Statistical significance was determined by two-way ANOVA with Dunnett multiple comparison test. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001; ns, not significant. Immunoblottings are shown beside each to determine the knockdown of target proteins and controls for virus infection.
    Ev D68 Us/Mo/14 18947 (Mo/47) Strain, supplied by Horng Gee Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    ev-d68 us/mo/14-18947 (mo/47) strain - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

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    HEK-293T cells were transfected with siRNA pools targeting eIF4A3, Y14, MAGOH, or non-targeting control siRNAs (NC), respectively. At 48 hrs post-transfection, cells were inoculated with the designated virus (A) HPIV3, (B) Cedar, (C) MuV, (D) NDV, (E) Influenza A (A/WSN/1933), (F) Enterovirus D68, and (G) SARA-CoV2 at a multiplicity of infection (m.o.i.) of 0.01. The titers of infectious supernatants were determined on Vero-CCL81 cells using a 10-fold serial dilution at the indicated time points. For each virus, the expression levels of endogenous eIF4AIII, Y14, and MAGOH, along with infection control for viral protein or EGFP reporter, were analyzed by immunoblotting; results shown beside each panel confirm the knockdown of target proteins and validate virus infection. Symbols represent the data points from biological triplicates. Bars represent the mean of the triplicates. Statistical significance was determined by two-way ANOVA with Dunnett multiple comparison test. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001; ns, not significant. Immunoblottings are shown beside each to determine the knockdown of target proteins and controls for virus infection.

    Journal: bioRxiv

    Article Title: Paramyxovirus matrix proteins modulate host cell translation via exon-junction complex interactions in the cytoplasm

    doi: 10.1101/2024.09.05.611502

    Figure Lengend Snippet: HEK-293T cells were transfected with siRNA pools targeting eIF4A3, Y14, MAGOH, or non-targeting control siRNAs (NC), respectively. At 48 hrs post-transfection, cells were inoculated with the designated virus (A) HPIV3, (B) Cedar, (C) MuV, (D) NDV, (E) Influenza A (A/WSN/1933), (F) Enterovirus D68, and (G) SARA-CoV2 at a multiplicity of infection (m.o.i.) of 0.01. The titers of infectious supernatants were determined on Vero-CCL81 cells using a 10-fold serial dilution at the indicated time points. For each virus, the expression levels of endogenous eIF4AIII, Y14, and MAGOH, along with infection control for viral protein or EGFP reporter, were analyzed by immunoblotting; results shown beside each panel confirm the knockdown of target proteins and validate virus infection. Symbols represent the data points from biological triplicates. Bars represent the mean of the triplicates. Statistical significance was determined by two-way ANOVA with Dunnett multiple comparison test. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001; ns, not significant. Immunoblottings are shown beside each to determine the knockdown of target proteins and controls for virus infection.

    Article Snippet: EV D68 strain US/MO/14-18947 (ATCC, VR-1823) were amplified in RD cells.

    Techniques: Transfection, Control, Virus, Infection, Serial Dilution, Expressing, Western Blot, Knockdown, Comparison